Recombinant Human PKLR Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032984-10, PKSH032984-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: PKLR

Target Synonym: Pyruvate Kinase Isozymes R/L; Pyruvate Kinase 1; R-Type/L-Type Pyruvate Kinase; Red Cell/Liver Pyruvate Kinase; PKLR; PK1; PKL

UNIProt ID: P30613

Research Areas: Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P30613

Background: Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.

Sequence: Met 1-Ser574

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT 0.2M NaCl, 10% glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.9 kDa

Observed MW: 58 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only